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1x mem non-essential amino acids solution (neaa)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1x mem non-essential amino acids solution (neaa)
    1x Mem Non Essential Amino Acids Solution (Neaa), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+mem+non-essential+amino+acids+solution+(neaa)/1%C3%97+mem+non+essential+amino+acids/pm40550888-195-22-35
    Average 90 stars, based on 1 article reviews
    1x mem non-essential amino acids solution (neaa) - by Bioz Stars, 2026-10
    90/100 stars

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    Control:

    Article Title: FAK modulates glioblastoma stem cell energetics via actomyosin contractility: regulation of glycolysis and mitochondrial function
    Article Snippet: NPE cells were cultured in Dulbecco’s Modified Eagle Medium and Ham’s Nutrient Mixture F12 (DMEM/F12; Sigma-Aldrich, #D8437) supplemented with 1.45 g/L D-Glucose (Sigma-Aldrich, #G8644), 120 μg/mL bovine serum albumin (BSA) fraction V solution (Gibco, #15260-037), 100 μM β-mercaptoethanol (Gibco; #31350-010), 1X MEM non-essential amino acid (MEM-NEAA) solution (Gibco; #11140-035), 0.5X B-27 (Gibco; #17504-044) supplement, 0.5X N-2 supplement (Gibco; #15140-122), 10 ng/mL murine EGF (Peprotech; #315-09), 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 μg/mL laminin-I (R&D Systems; #3446-005-01).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: PD-L1 ( CD274 ) promoter hypomethylation predicts immunotherapy response in metastatic urothelial carcinoma
    Article Snippet: S 0615HI, Bio&SELL GmbH, Nuremberg, Germany), 1X MEM (Minimum Essential Medium) Non-Essential Amino Acids Solution (100X stock, cat. no. 11140035, Thermo Fisher Scientific), 1 mM sodium pyruvate (100 mM stock, cat. no. 11360070, Thermo Fisher Scientific), and 100 U/ml penicillin and streptomycin (10,000 U/ml stock, cat. no. 15140122, Thermo Fisher Scientific).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations
    Article Snippet: The cells were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco), for Huh7.5.1 cells, the medium was also supplemented with 1X MEM Non‐Essential Amino Acids Solution (MEM NEAA, Gibco), all cells were cultured at 37 °C and 5% CO 2 .

    Article Title: PHD2 safeguards modest mesendoderm development
    Article Snippet: AB2.2 and R1 mESCs were cultured with Knock Out DMEM (Gibco, Cat No. 10829018) supplemented with 15% fetal bovine serum (FBS, Excell Bio, Cat No. FND500), 1x MEM Non-Essential Amino Acids Solution (NEAA, Gibco, Cat No. 11140050), 2 mM L-Glutamine (Gibco, Cat No. 25030081), 50 U/ml Penicillin, 50 μg/ml Streptomycin (Gibco, Cat No. 15240062), 0.1 mM 2-Mercaptoethanol (Merck, Cat No. M3148), 3 μM CHIR99021(MCE, Cat No. HY-10182), 1 μM PD0325901 (MCE, Cat No. HY-10254), and 10 3 units of LIF (Millipore, Cat No. ESG1107) on 0.1% Gelatin (Sigma, Cat No. G1890)-coated cell culture dishes.

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis
    Article Snippet: For organoids experiment, after 2 or 3 days of seeding in Matrigel, CB839 (Selleckchem) was used in different concentrations (1 μM, 5 μM, 10 μM, 20 μM) to inhibit GLS1.

    Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration
    Article Snippet: The pelleted cells were resuspended in pre-differentiation neuronal media, composed of KnockOut DMEM/F12 (Thermo Fisher Scientific; 12660012) supplemented with 1X N2 supplement (Thermo Fisher Scientific; 17502048), 1X MEM Non-Essential Amino Acids Solution (NEAA) (Thermo Fisher Scientific; 11140050), 1X GlutaMAX Supplement (Thermo Fisher Scientific; 35050061), 1mg/ml Laminin (Thermo Fisher Scientific; 23017015) and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation. iPSCs were plated 4×10 cells per Matrigel-coated well of a 6-well plate in 2 ml of pre-differentiation neuronal media.

    Incubation:

    Article Title: FAK modulates glioblastoma stem cell energetics via actomyosin contractility: regulation of glycolysis and mitochondrial function
    Article Snippet: NPE cells were cultured in Dulbecco’s Modified Eagle Medium and Ham’s Nutrient Mixture F12 (DMEM/F12; Sigma-Aldrich, #D8437) supplemented with 1.45 g/L D-Glucose (Sigma-Aldrich, #G8644), 120 μg/mL bovine serum albumin (BSA) fraction V solution (Gibco, #15260-037), 100 μM β-mercaptoethanol (Gibco; #31350-010), 1X MEM non-essential amino acid (MEM-NEAA) solution (Gibco; #11140-035), 0.5X B-27 (Gibco; #17504-044) supplement, 0.5X N-2 supplement (Gibco; #15140-122), 10 ng/mL murine EGF (Peprotech; #315-09), 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 μg/mL laminin-I (R&D Systems; #3446-005-01).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: PD-L1 ( CD274 ) promoter hypomethylation predicts immunotherapy response in metastatic urothelial carcinoma
    Article Snippet: S 0615HI, Bio&SELL GmbH, Nuremberg, Germany), 1X MEM (Minimum Essential Medium) Non-Essential Amino Acids Solution (100X stock, cat. no. 11140035, Thermo Fisher Scientific), 1 mM sodium pyruvate (100 mM stock, cat. no. 11360070, Thermo Fisher Scientific), and 100 U/ml penicillin and streptomycin (10,000 U/ml stock, cat. no. 15140122, Thermo Fisher Scientific).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations
    Article Snippet: The cells were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco), for Huh7.5.1 cells, the medium was also supplemented with 1X MEM Non‐Essential Amino Acids Solution (MEM NEAA, Gibco), all cells were cultured at 37 °C and 5% CO 2 .

    Article Title: PHD2 safeguards modest mesendoderm development
    Article Snippet: AB2.2 and R1 mESCs were cultured with Knock Out DMEM (Gibco, Cat No. 10829018) supplemented with 15% fetal bovine serum (FBS, Excell Bio, Cat No. FND500), 1x MEM Non-Essential Amino Acids Solution (NEAA, Gibco, Cat No. 11140050), 2 mM L-Glutamine (Gibco, Cat No. 25030081), 50 U/ml Penicillin, 50 μg/ml Streptomycin (Gibco, Cat No. 15240062), 0.1 mM 2-Mercaptoethanol (Merck, Cat No. M3148), 3 μM CHIR99021(MCE, Cat No. HY-10182), 1 μM PD0325901 (MCE, Cat No. HY-10254), and 10 3 units of LIF (Millipore, Cat No. ESG1107) on 0.1% Gelatin (Sigma, Cat No. G1890)-coated cell culture dishes.

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis
    Article Snippet: For organoids experiment, after 2 or 3 days of seeding in Matrigel, CB839 (Selleckchem) was used in different concentrations (1 μM, 5 μM, 10 μM, 20 μM) to inhibit GLS1.

    Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration
    Article Snippet: The pelleted cells were resuspended in pre-differentiation neuronal media, composed of KnockOut DMEM/F12 (Thermo Fisher Scientific; 12660012) supplemented with 1X N2 supplement (Thermo Fisher Scientific; 17502048), 1X MEM Non-Essential Amino Acids Solution (NEAA) (Thermo Fisher Scientific; 11140050), 1X GlutaMAX Supplement (Thermo Fisher Scientific; 35050061), 1mg/ml Laminin (Thermo Fisher Scientific; 23017015) and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation. iPSCs were plated 4×10 cells per Matrigel-coated well of a 6-well plate in 2 ml of pre-differentiation neuronal media.

    Fluorescence:

    Article Title: FAK modulates glioblastoma stem cell energetics via actomyosin contractility: regulation of glycolysis and mitochondrial function
    Article Snippet: NPE cells were cultured in Dulbecco’s Modified Eagle Medium and Ham’s Nutrient Mixture F12 (DMEM/F12; Sigma-Aldrich, #D8437) supplemented with 1.45 g/L D-Glucose (Sigma-Aldrich, #G8644), 120 μg/mL bovine serum albumin (BSA) fraction V solution (Gibco, #15260-037), 100 μM β-mercaptoethanol (Gibco; #31350-010), 1X MEM non-essential amino acid (MEM-NEAA) solution (Gibco; #11140-035), 0.5X B-27 (Gibco; #17504-044) supplement, 0.5X N-2 supplement (Gibco; #15140-122), 10 ng/mL murine EGF (Peprotech; #315-09), 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 μg/mL laminin-I (R&D Systems; #3446-005-01).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: PD-L1 ( CD274 ) promoter hypomethylation predicts immunotherapy response in metastatic urothelial carcinoma
    Article Snippet: S 0615HI, Bio&SELL GmbH, Nuremberg, Germany), 1X MEM (Minimum Essential Medium) Non-Essential Amino Acids Solution (100X stock, cat. no. 11140035, Thermo Fisher Scientific), 1 mM sodium pyruvate (100 mM stock, cat. no. 11360070, Thermo Fisher Scientific), and 100 U/ml penicillin and streptomycin (10,000 U/ml stock, cat. no. 15140122, Thermo Fisher Scientific).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations
    Article Snippet: The cells were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco), for Huh7.5.1 cells, the medium was also supplemented with 1X MEM Non‐Essential Amino Acids Solution (MEM NEAA, Gibco), all cells were cultured at 37 °C and 5% CO 2 .

    Article Title: PHD2 safeguards modest mesendoderm development
    Article Snippet: AB2.2 and R1 mESCs were cultured with Knock Out DMEM (Gibco, Cat No. 10829018) supplemented with 15% fetal bovine serum (FBS, Excell Bio, Cat No. FND500), 1x MEM Non-Essential Amino Acids Solution (NEAA, Gibco, Cat No. 11140050), 2 mM L-Glutamine (Gibco, Cat No. 25030081), 50 U/ml Penicillin, 50 μg/ml Streptomycin (Gibco, Cat No. 15240062), 0.1 mM 2-Mercaptoethanol (Merck, Cat No. M3148), 3 μM CHIR99021(MCE, Cat No. HY-10182), 1 μM PD0325901 (MCE, Cat No. HY-10254), and 10 3 units of LIF (Millipore, Cat No. ESG1107) on 0.1% Gelatin (Sigma, Cat No. G1890)-coated cell culture dishes.

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis
    Article Snippet: For organoids experiment, after 2 or 3 days of seeding in Matrigel, CB839 (Selleckchem) was used in different concentrations (1 μM, 5 μM, 10 μM, 20 μM) to inhibit GLS1.

    Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration
    Article Snippet: The pelleted cells were resuspended in pre-differentiation neuronal media, composed of KnockOut DMEM/F12 (Thermo Fisher Scientific; 12660012) supplemented with 1X N2 supplement (Thermo Fisher Scientific; 17502048), 1X MEM Non-Essential Amino Acids Solution (NEAA) (Thermo Fisher Scientific; 11140050), 1X GlutaMAX Supplement (Thermo Fisher Scientific; 35050061), 1mg/ml Laminin (Thermo Fisher Scientific; 23017015) and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation. iPSCs were plated 4×10 cells per Matrigel-coated well of a 6-well plate in 2 ml of pre-differentiation neuronal media.

    BrdU Incorporation Assay:

    Article Title: FAK modulates glioblastoma stem cell energetics via actomyosin contractility: regulation of glycolysis and mitochondrial function
    Article Snippet: NPE cells were cultured in Dulbecco’s Modified Eagle Medium and Ham’s Nutrient Mixture F12 (DMEM/F12; Sigma-Aldrich, #D8437) supplemented with 1.45 g/L D-Glucose (Sigma-Aldrich, #G8644), 120 μg/mL bovine serum albumin (BSA) fraction V solution (Gibco, #15260-037), 100 μM β-mercaptoethanol (Gibco; #31350-010), 1X MEM non-essential amino acid (MEM-NEAA) solution (Gibco; #11140-035), 0.5X B-27 (Gibco; #17504-044) supplement, 0.5X N-2 supplement (Gibco; #15140-122), 10 ng/mL murine EGF (Peprotech; #315-09), 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 μg/mL laminin-I (R&D Systems; #3446-005-01).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: PD-L1 ( CD274 ) promoter hypomethylation predicts immunotherapy response in metastatic urothelial carcinoma
    Article Snippet: S 0615HI, Bio&SELL GmbH, Nuremberg, Germany), 1X MEM (Minimum Essential Medium) Non-Essential Amino Acids Solution (100X stock, cat. no. 11140035, Thermo Fisher Scientific), 1 mM sodium pyruvate (100 mM stock, cat. no. 11360070, Thermo Fisher Scientific), and 100 U/ml penicillin and streptomycin (10,000 U/ml stock, cat. no. 15140122, Thermo Fisher Scientific).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations
    Article Snippet: The cells were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco), for Huh7.5.1 cells, the medium was also supplemented with 1X MEM Non‐Essential Amino Acids Solution (MEM NEAA, Gibco), all cells were cultured at 37 °C and 5% CO 2 .

    Article Title: PHD2 safeguards modest mesendoderm development
    Article Snippet: AB2.2 and R1 mESCs were cultured with Knock Out DMEM (Gibco, Cat No. 10829018) supplemented with 15% fetal bovine serum (FBS, Excell Bio, Cat No. FND500), 1x MEM Non-Essential Amino Acids Solution (NEAA, Gibco, Cat No. 11140050), 2 mM L-Glutamine (Gibco, Cat No. 25030081), 50 U/ml Penicillin, 50 μg/ml Streptomycin (Gibco, Cat No. 15240062), 0.1 mM 2-Mercaptoethanol (Merck, Cat No. M3148), 3 μM CHIR99021(MCE, Cat No. HY-10182), 1 μM PD0325901 (MCE, Cat No. HY-10254), and 10 3 units of LIF (Millipore, Cat No. ESG1107) on 0.1% Gelatin (Sigma, Cat No. G1890)-coated cell culture dishes.

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis
    Article Snippet: For organoids experiment, after 2 or 3 days of seeding in Matrigel, CB839 (Selleckchem) was used in different concentrations (1 μM, 5 μM, 10 μM, 20 μM) to inhibit GLS1.

    Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration
    Article Snippet: The pelleted cells were resuspended in pre-differentiation neuronal media, composed of KnockOut DMEM/F12 (Thermo Fisher Scientific; 12660012) supplemented with 1X N2 supplement (Thermo Fisher Scientific; 17502048), 1X MEM Non-Essential Amino Acids Solution (NEAA) (Thermo Fisher Scientific; 11140050), 1X GlutaMAX Supplement (Thermo Fisher Scientific; 35050061), 1mg/ml Laminin (Thermo Fisher Scientific; 23017015) and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation. iPSCs were plated 4×10 cells per Matrigel-coated well of a 6-well plate in 2 ml of pre-differentiation neuronal media.

    Translocation Assay:

    Article Title: FAK modulates glioblastoma stem cell energetics via actomyosin contractility: regulation of glycolysis and mitochondrial function
    Article Snippet: NPE cells were cultured in Dulbecco’s Modified Eagle Medium and Ham’s Nutrient Mixture F12 (DMEM/F12; Sigma-Aldrich, #D8437) supplemented with 1.45 g/L D-Glucose (Sigma-Aldrich, #G8644), 120 μg/mL bovine serum albumin (BSA) fraction V solution (Gibco, #15260-037), 100 μM β-mercaptoethanol (Gibco; #31350-010), 1X MEM non-essential amino acid (MEM-NEAA) solution (Gibco; #11140-035), 0.5X B-27 (Gibco; #17504-044) supplement, 0.5X N-2 supplement (Gibco; #15140-122), 10 ng/mL murine EGF (Peprotech; #315-09), 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 μg/mL laminin-I (R&D Systems; #3446-005-01).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: PD-L1 ( CD274 ) promoter hypomethylation predicts immunotherapy response in metastatic urothelial carcinoma
    Article Snippet: S 0615HI, Bio&SELL GmbH, Nuremberg, Germany), 1X MEM (Minimum Essential Medium) Non-Essential Amino Acids Solution (100X stock, cat. no. 11140035, Thermo Fisher Scientific), 1 mM sodium pyruvate (100 mM stock, cat. no. 11360070, Thermo Fisher Scientific), and 100 U/ml penicillin and streptomycin (10,000 U/ml stock, cat. no. 15140122, Thermo Fisher Scientific).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations
    Article Snippet: The cells were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco), for Huh7.5.1 cells, the medium was also supplemented with 1X MEM Non‐Essential Amino Acids Solution (MEM NEAA, Gibco), all cells were cultured at 37 °C and 5% CO 2 .

    Article Title: PHD2 safeguards modest mesendoderm development
    Article Snippet: AB2.2 and R1 mESCs were cultured with Knock Out DMEM (Gibco, Cat No. 10829018) supplemented with 15% fetal bovine serum (FBS, Excell Bio, Cat No. FND500), 1x MEM Non-Essential Amino Acids Solution (NEAA, Gibco, Cat No. 11140050), 2 mM L-Glutamine (Gibco, Cat No. 25030081), 50 U/ml Penicillin, 50 μg/ml Streptomycin (Gibco, Cat No. 15240062), 0.1 mM 2-Mercaptoethanol (Merck, Cat No. M3148), 3 μM CHIR99021(MCE, Cat No. HY-10182), 1 μM PD0325901 (MCE, Cat No. HY-10254), and 10 3 units of LIF (Millipore, Cat No. ESG1107) on 0.1% Gelatin (Sigma, Cat No. G1890)-coated cell culture dishes.

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis
    Article Snippet: For organoids experiment, after 2 or 3 days of seeding in Matrigel, CB839 (Selleckchem) was used in different concentrations (1 μM, 5 μM, 10 μM, 20 μM) to inhibit GLS1.

    Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration
    Article Snippet: The pelleted cells were resuspended in pre-differentiation neuronal media, composed of KnockOut DMEM/F12 (Thermo Fisher Scientific; 12660012) supplemented with 1X N2 supplement (Thermo Fisher Scientific; 17502048), 1X MEM Non-Essential Amino Acids Solution (NEAA) (Thermo Fisher Scientific; 11140050), 1X GlutaMAX Supplement (Thermo Fisher Scientific; 35050061), 1mg/ml Laminin (Thermo Fisher Scientific; 23017015) and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation. iPSCs were plated 4×10 cells per Matrigel-coated well of a 6-well plate in 2 ml of pre-differentiation neuronal media.

    Cell Culture:

    Article Title: FAK modulates glioblastoma stem cell energetics via actomyosin contractility: regulation of glycolysis and mitochondrial function
    Article Snippet: NPE cells were cultured in Dulbecco’s Modified Eagle Medium and Ham’s Nutrient Mixture F12 (DMEM/F12; Sigma-Aldrich, #D8437) supplemented with 1.45 g/L D-Glucose (Sigma-Aldrich, #G8644), 120 μg/mL bovine serum albumin (BSA) fraction V solution (Gibco, #15260-037), 100 μM β-mercaptoethanol (Gibco; #31350-010), 1X MEM non-essential amino acid (MEM-NEAA) solution (Gibco; #11140-035), 0.5X B-27 (Gibco; #17504-044) supplement, 0.5X N-2 supplement (Gibco; #15140-122), 10 ng/mL murine EGF (Peprotech; #315-09), 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 μg/mL laminin-I (R&D Systems; #3446-005-01).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: PD-L1 ( CD274 ) promoter hypomethylation predicts immunotherapy response in metastatic urothelial carcinoma
    Article Snippet: S 0615HI, Bio&SELL GmbH, Nuremberg, Germany), 1X MEM (Minimum Essential Medium) Non-Essential Amino Acids Solution (100X stock, cat. no. 11140035, Thermo Fisher Scientific), 1 mM sodium pyruvate (100 mM stock, cat. no. 11360070, Thermo Fisher Scientific), and 100 U/ml penicillin and streptomycin (10,000 U/ml stock, cat. no. 15140122, Thermo Fisher Scientific).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations
    Article Snippet: The cells were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco), for Huh7.5.1 cells, the medium was also supplemented with 1X MEM Non‐Essential Amino Acids Solution (MEM NEAA, Gibco), all cells were cultured at 37 °C and 5% CO 2 .

    Article Title: PHD2 safeguards modest mesendoderm development
    Article Snippet: AB2.2 and R1 mESCs were cultured with Knock Out DMEM (Gibco, Cat No. 10829018) supplemented with 15% fetal bovine serum (FBS, Excell Bio, Cat No. FND500), 1x MEM Non-Essential Amino Acids Solution (NEAA, Gibco, Cat No. 11140050), 2 mM L-Glutamine (Gibco, Cat No. 25030081), 50 U/ml Penicillin, 50 μg/ml Streptomycin (Gibco, Cat No. 15240062), 0.1 mM 2-Mercaptoethanol (Merck, Cat No. M3148), 3 μM CHIR99021(MCE, Cat No. HY-10182), 1 μM PD0325901 (MCE, Cat No. HY-10254), and 10 3 units of LIF (Millipore, Cat No. ESG1107) on 0.1% Gelatin (Sigma, Cat No. G1890)-coated cell culture dishes.

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis
    Article Snippet: For organoids experiment, after 2 or 3 days of seeding in Matrigel, CB839 (Selleckchem) was used in different concentrations (1 μM, 5 μM, 10 μM, 20 μM) to inhibit GLS1.

    Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration
    Article Snippet: The pelleted cells were resuspended in pre-differentiation neuronal media, composed of KnockOut DMEM/F12 (Thermo Fisher Scientific; 12660012) supplemented with 1X N2 supplement (Thermo Fisher Scientific; 17502048), 1X MEM Non-Essential Amino Acids Solution (NEAA) (Thermo Fisher Scientific; 11140050), 1X GlutaMAX Supplement (Thermo Fisher Scientific; 35050061), 1mg/ml Laminin (Thermo Fisher Scientific; 23017015) and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation. iPSCs were plated 4×10 cells per Matrigel-coated well of a 6-well plate in 2 ml of pre-differentiation neuronal media.

    Modification:

    Article Title: FAK modulates glioblastoma stem cell energetics via actomyosin contractility: regulation of glycolysis and mitochondrial function
    Article Snippet: NPE cells were cultured in Dulbecco’s Modified Eagle Medium and Ham’s Nutrient Mixture F12 (DMEM/F12; Sigma-Aldrich, #D8437) supplemented with 1.45 g/L D-Glucose (Sigma-Aldrich, #G8644), 120 μg/mL bovine serum albumin (BSA) fraction V solution (Gibco, #15260-037), 100 μM β-mercaptoethanol (Gibco; #31350-010), 1X MEM non-essential amino acid (MEM-NEAA) solution (Gibco; #11140-035), 0.5X B-27 (Gibco; #17504-044) supplement, 0.5X N-2 supplement (Gibco; #15140-122), 10 ng/mL murine EGF (Peprotech; #315-09), 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 μg/mL laminin-I (R&D Systems; #3446-005-01).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: PD-L1 ( CD274 ) promoter hypomethylation predicts immunotherapy response in metastatic urothelial carcinoma
    Article Snippet: S 0615HI, Bio&SELL GmbH, Nuremberg, Germany), 1X MEM (Minimum Essential Medium) Non-Essential Amino Acids Solution (100X stock, cat. no. 11140035, Thermo Fisher Scientific), 1 mM sodium pyruvate (100 mM stock, cat. no. 11360070, Thermo Fisher Scientific), and 100 U/ml penicillin and streptomycin (10,000 U/ml stock, cat. no. 15140122, Thermo Fisher Scientific).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations
    Article Snippet: The cells were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco), for Huh7.5.1 cells, the medium was also supplemented with 1X MEM Non‐Essential Amino Acids Solution (MEM NEAA, Gibco), all cells were cultured at 37 °C and 5% CO 2 .

    Article Title: PHD2 safeguards modest mesendoderm development
    Article Snippet: AB2.2 and R1 mESCs were cultured with Knock Out DMEM (Gibco, Cat No. 10829018) supplemented with 15% fetal bovine serum (FBS, Excell Bio, Cat No. FND500), 1x MEM Non-Essential Amino Acids Solution (NEAA, Gibco, Cat No. 11140050), 2 mM L-Glutamine (Gibco, Cat No. 25030081), 50 U/ml Penicillin, 50 μg/ml Streptomycin (Gibco, Cat No. 15240062), 0.1 mM 2-Mercaptoethanol (Merck, Cat No. M3148), 3 μM CHIR99021(MCE, Cat No. HY-10182), 1 μM PD0325901 (MCE, Cat No. HY-10254), and 10 3 units of LIF (Millipore, Cat No. ESG1107) on 0.1% Gelatin (Sigma, Cat No. G1890)-coated cell culture dishes.

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis
    Article Snippet: For organoids experiment, after 2 or 3 days of seeding in Matrigel, CB839 (Selleckchem) was used in different concentrations (1 μM, 5 μM, 10 μM, 20 μM) to inhibit GLS1.

    Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration
    Article Snippet: The pelleted cells were resuspended in pre-differentiation neuronal media, composed of KnockOut DMEM/F12 (Thermo Fisher Scientific; 12660012) supplemented with 1X N2 supplement (Thermo Fisher Scientific; 17502048), 1X MEM Non-Essential Amino Acids Solution (NEAA) (Thermo Fisher Scientific; 11140050), 1X GlutaMAX Supplement (Thermo Fisher Scientific; 35050061), 1mg/ml Laminin (Thermo Fisher Scientific; 23017015) and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation. iPSCs were plated 4×10 cells per Matrigel-coated well of a 6-well plate in 2 ml of pre-differentiation neuronal media.

    Glo Assay:

    Article Title: FAK modulates glioblastoma stem cell energetics via actomyosin contractility: regulation of glycolysis and mitochondrial function
    Article Snippet: NPE cells were cultured in Dulbecco’s Modified Eagle Medium and Ham’s Nutrient Mixture F12 (DMEM/F12; Sigma-Aldrich, #D8437) supplemented with 1.45 g/L D-Glucose (Sigma-Aldrich, #G8644), 120 μg/mL bovine serum albumin (BSA) fraction V solution (Gibco, #15260-037), 100 μM β-mercaptoethanol (Gibco; #31350-010), 1X MEM non-essential amino acid (MEM-NEAA) solution (Gibco; #11140-035), 0.5X B-27 (Gibco; #17504-044) supplement, 0.5X N-2 supplement (Gibco; #15140-122), 10 ng/mL murine EGF (Peprotech; #315-09), 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 μg/mL laminin-I (R&D Systems; #3446-005-01).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: PD-L1 ( CD274 ) promoter hypomethylation predicts immunotherapy response in metastatic urothelial carcinoma
    Article Snippet: S 0615HI, Bio&SELL GmbH, Nuremberg, Germany), 1X MEM (Minimum Essential Medium) Non-Essential Amino Acids Solution (100X stock, cat. no. 11140035, Thermo Fisher Scientific), 1 mM sodium pyruvate (100 mM stock, cat. no. 11360070, Thermo Fisher Scientific), and 100 U/ml penicillin and streptomycin (10,000 U/ml stock, cat. no. 15140122, Thermo Fisher Scientific).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations
    Article Snippet: The cells were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco), for Huh7.5.1 cells, the medium was also supplemented with 1X MEM Non‐Essential Amino Acids Solution (MEM NEAA, Gibco), all cells were cultured at 37 °C and 5% CO 2 .

    Article Title: PHD2 safeguards modest mesendoderm development
    Article Snippet: AB2.2 and R1 mESCs were cultured with Knock Out DMEM (Gibco, Cat No. 10829018) supplemented with 15% fetal bovine serum (FBS, Excell Bio, Cat No. FND500), 1x MEM Non-Essential Amino Acids Solution (NEAA, Gibco, Cat No. 11140050), 2 mM L-Glutamine (Gibco, Cat No. 25030081), 50 U/ml Penicillin, 50 μg/ml Streptomycin (Gibco, Cat No. 15240062), 0.1 mM 2-Mercaptoethanol (Merck, Cat No. M3148), 3 μM CHIR99021(MCE, Cat No. HY-10182), 1 μM PD0325901 (MCE, Cat No. HY-10254), and 10 3 units of LIF (Millipore, Cat No. ESG1107) on 0.1% Gelatin (Sigma, Cat No. G1890)-coated cell culture dishes.

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis
    Article Snippet: For organoids experiment, after 2 or 3 days of seeding in Matrigel, CB839 (Selleckchem) was used in different concentrations (1 μM, 5 μM, 10 μM, 20 μM) to inhibit GLS1.

    Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration
    Article Snippet: The pelleted cells were resuspended in pre-differentiation neuronal media, composed of KnockOut DMEM/F12 (Thermo Fisher Scientific; 12660012) supplemented with 1X N2 supplement (Thermo Fisher Scientific; 17502048), 1X MEM Non-Essential Amino Acids Solution (NEAA) (Thermo Fisher Scientific; 11140050), 1X GlutaMAX Supplement (Thermo Fisher Scientific; 35050061), 1mg/ml Laminin (Thermo Fisher Scientific; 23017015) and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation. iPSCs were plated 4×10 cells per Matrigel-coated well of a 6-well plate in 2 ml of pre-differentiation neuronal media.

    Knock-Out:

    Article Title: FAK modulates glioblastoma stem cell energetics via actomyosin contractility: regulation of glycolysis and mitochondrial function
    Article Snippet: NPE cells were cultured in Dulbecco’s Modified Eagle Medium and Ham’s Nutrient Mixture F12 (DMEM/F12; Sigma-Aldrich, #D8437) supplemented with 1.45 g/L D-Glucose (Sigma-Aldrich, #G8644), 120 μg/mL bovine serum albumin (BSA) fraction V solution (Gibco, #15260-037), 100 μM β-mercaptoethanol (Gibco; #31350-010), 1X MEM non-essential amino acid (MEM-NEAA) solution (Gibco; #11140-035), 0.5X B-27 (Gibco; #17504-044) supplement, 0.5X N-2 supplement (Gibco; #15140-122), 10 ng/mL murine EGF (Peprotech; #315-09), 10 ng/mL human b-FGF (Peprotech; #100-18b), and 1 μg/mL laminin-I (R&D Systems; #3446-005-01).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: PD-L1 ( CD274 ) promoter hypomethylation predicts immunotherapy response in metastatic urothelial carcinoma
    Article Snippet: S 0615HI, Bio&SELL GmbH, Nuremberg, Germany), 1X MEM (Minimum Essential Medium) Non-Essential Amino Acids Solution (100X stock, cat. no. 11140035, Thermo Fisher Scientific), 1 mM sodium pyruvate (100 mM stock, cat. no. 11360070, Thermo Fisher Scientific), and 100 U/ml penicillin and streptomycin (10,000 U/ml stock, cat. no. 15140122, Thermo Fisher Scientific).

    Article Title: Potent and Selective SETDB1 Covalent Negative Allosteric Modulator Reduces Methyltransferase Activity in Cells
    Article Snippet: MCF-7 cells were obtained from the UNC Lineberger Tissue Culture facility and were cultured in DMEM (Sigma or Corning) supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin (Sigma), 0.01 mg/mL of insulin from bovine pancreas (Sigma), with (Cell Titer Glo assay) or without (ABPP experiment) 1X Minimum Essential Medium Non-Essential Amino Acids Solution (Gibco).

    Article Title: A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations
    Article Snippet: The cells were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin and streptomycin (Gibco), for Huh7.5.1 cells, the medium was also supplemented with 1X MEM Non‐Essential Amino Acids Solution (MEM NEAA, Gibco), all cells were cultured at 37 °C and 5% CO 2 .

    Article Title: PHD2 safeguards modest mesendoderm development
    Article Snippet: AB2.2 and R1 mESCs were cultured with Knock Out DMEM (Gibco, Cat No. 10829018) supplemented with 15% fetal bovine serum (FBS, Excell Bio, Cat No. FND500), 1x MEM Non-Essential Amino Acids Solution (NEAA, Gibco, Cat No. 11140050), 2 mM L-Glutamine (Gibco, Cat No. 25030081), 50 U/ml Penicillin, 50 μg/ml Streptomycin (Gibco, Cat No. 15240062), 0.1 mM 2-Mercaptoethanol (Merck, Cat No. M3148), 3 μM CHIR99021(MCE, Cat No. HY-10182), 1 μM PD0325901 (MCE, Cat No. HY-10254), and 10 3 units of LIF (Millipore, Cat No. ESG1107) on 0.1% Gelatin (Sigma, Cat No. G1890)-coated cell culture dishes.

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis
    Article Snippet: For organoids experiment, after 2 or 3 days of seeding in Matrigel, CB839 (Selleckchem) was used in different concentrations (1 μM, 5 μM, 10 μM, 20 μM) to inhibit GLS1.

    Article Title: Presynapses are mitophagy pit stops that prevent axon degeneration
    Article Snippet: The pelleted cells were resuspended in pre-differentiation neuronal media, composed of KnockOut DMEM/F12 (Thermo Fisher Scientific; 12660012) supplemented with 1X N2 supplement (Thermo Fisher Scientific; 17502048), 1X MEM Non-Essential Amino Acids Solution (NEAA) (Thermo Fisher Scientific; 11140050), 1X GlutaMAX Supplement (Thermo Fisher Scientific; 35050061), 1mg/ml Laminin (Thermo Fisher Scientific; 23017015) and freshly added 10 mM ROCK inhibitor and 2 mg/ml Doxycycline (Sigma, D9891-5) to initiate differentiation. iPSCs were plated 4×10 cells per Matrigel-coated well of a 6-well plate in 2 ml of pre-differentiation neuronal media.



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